[实验研究]电针对便秘型肠易激综合征模型大鼠血浆降钙素基因相关肽和P物质表达的影响

Time:2016-02-22 00:05:56 来源:250355 济南,山东中医药大学基础医学院病理教研室(张亚楠、王晓燕),2010级博士研究生(张亚楠);山东中医药大学国际教育学院(王世军) 等 作者:张亚楠 王世军 王晓燕

《国际中医中药杂志》[ISSN:1673-4246/CN:CN11-5398/R]

卷: 35 期数: 2013年05期

页码: 413-415

栏目: 实验研究

出版日期: 2013-05-05

文章信息/Info

Title: Effects of electroacupuncture on plasma CGRP and SP expression in rats of constipation-predominant irritable bowel syndrome

作者: 张亚楠 王世军 王晓燕 250355 济南,山东中医药大学基础医学院病理教研室(张亚楠、王晓燕),2010级博士研究生(张亚楠);山东中医药大学国际教育学院(王世军)

Author(s): ZHANG Ya-nan*;  WANG Shi-jun;  WANG Xiao-yan *International eucation clleage of Shandong uiversity of taditional Chinese mdicine, Jinan 250355, China

关键词: 电针; 肠易激综合症; 降钙素基因相关肽; P物质

Keywords: Electro-acupuncture; Irritable bowel syndrome; Calcitonin gene-related peptide; Substance P

DOI: 10.3760/cma.j.issn.1673-4246.2013.05.010

摘要: 目的 观察电针对便秘型肠易激综合征大鼠血浆中降钙素基因相关肽(CGRP)、P物质(SP)表达的影响,探讨电针治疗肠易激综合征的作用机制。方法 采用随机数字表法将32只Wistar成年雄性大鼠随机分为正常对照组、IBS模型组、电针上巨虚组、电针大肠俞组,每组8只,采用冰水灌胃法造模14 d。从造模成功后第1天起,电针上巨虚组、电针大肠腧组采用电针治疗,1次/d,连续7 d。正常组不做任何处理。治疗结束后,麻醉状态下取下腔静脉血5 ml,采用酶联免疫法(Elisa)检测大鼠血浆中CGRP、SP的含量。结果 与对照正常组比较,IBS模型组大鼠CGRP、SP含量[分别为(52.62±15.05)pg/ml、(27.10±5.03)pg/ml]明显升高,差异有统计学意义(P均<0.01);电针上巨虚组、电针大肠俞组CGRP含量[分别为(37.82±15.34)pg/ml、(38.93±12.05)pg/ml]、SP含量[分别为(19.62±5.68)pg/ml、(18.90±5.07)pg/ml]变化均不明显(P均>0.05)。与IBS模型组比较,电针上巨虚组、电针大肠俞组CGRP含量明显降低(P均>0.05)、SP含量明显降低(P均<0.01)。电针上巨虚组与电针大肠俞组比较,CGRP、SP含量相当,无统计学意义(P均>0.05)。结论 电针可通过调节便秘型肠易激综合征模型大鼠的CGRP、SP发挥治疗作用。

Abstract: Objective To observe effects of electro-acupuncture on the expression of calcitonin gene-related peptide(CGRP) and substance P(SP) in constipation-predominant IBS rats, and to discuss the mechanism of IBS’s acupuncture treatment. Methods To set WISTAR adult rats as the object and to establish models of irritable bowel syndrome rats. The experiment rats were randomly divided into a normal group, a model group, a EA Shangjuxu group and a EA Dachangshu group. The Shangjuxu group and Dachangshu group were bounded and given EA, once daily, for a total of seven times. Model group was bounded but not treated with EA , the normal group was done nothing. After the treatment, 5ml of inferior vena blood was got in anesthesia state. The value of plasma CGRP and SP in rats’ plasma were measued with Elisa. Results Compared with the normal control group, CGRP and SP content[(52.62±15.05)pg/ml, (27.10±5.03)pg/ml] in IBS model group was obviously increased, the difference was statistically significant(P<0.01, P<0.01); the changes of plasma CGRP content [(37.82±15.34)pg/ml, (38.93±12.05)pg/ml] and SP content[(19.62±5.68)pg/ml, (18.90±5.07)pg/ml] were not obvious in EA Shangjuxu group and EA Dachangshu group(P>0.05, P>0.05). Compared with IBS model group, the content of plasma CGRP and SP was significantly descend in EA Shangjuxu group and EA Dachangshu group(P<0.05, P<0.05, P<0.01, P<0.01). The concentration of CGRP and SP in EA Shangjuxu group and EA Dachangshu group had no significant difference(P>0.05, P>0.05). Conclusion Electroacupuncture could treat IBS through regulating CGRP and SP in IBS rats.

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