[论著]不同产地昆明山海棠对巨噬细胞炎性因子的影响

Time:2016-02-22 00:37:25 来源:100050 首都医科大学附属北京友谊医院中药剂科(续畅、赵奎君、马致洁);100039北京,中国人民解放军第三〇二医院全军中医药研究所(赵庆国、肖小河、王伽伯)等 作者:续畅 赵庆国 肖小河 赵奎君 王伽伯 马致洁

《国际中医中药杂志》[ISSN:1673-4246/CN:CN11-5398/R]

卷: 37 期数: 2015年11期

页码: 1005-1009

栏目: 论著

出版日期: 2015-11-05

文章信息/Info

Title: The effect of different regions of Tripterygium Hypoglaucum (Lévl.) Hutch on macrophage inflammatory factor

作者: 续畅 赵庆国 肖小河 赵奎君 王伽伯 马致洁 100050 首都医科大学附属北京友谊医院中药剂科(续畅、赵奎君、马致洁);100039北京,中国人民解放军第三〇二医院全军中医药研究所(赵庆国、肖小河、王伽伯)

Author(s): Xu Chang' target="_blank" rel="external"> Xu Chang*;  Zhao Qingguo;  Xiao Xiaohe;  Zhao Kuijun;  Wang Jiabo; ' target="_blank" rel="external"> Ma Zhijie. * Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China

关键词: 昆明山海棠; 巨噬细胞; 关节炎; 类风湿; 地道药材

Keywords: Tripterygium Hypoglaucum (Lévl.) Hutch' target="_blank" rel="external">Tripterygium Hypoglaucum (Lévl.) Hutch;  Macrophage;  Arthritis;  rheumatoid;  Genuine crude drug

DOI: 10.3760/cma.j.issn.1673-4246.2015.11.012

摘要:

目的 观察不同产地昆明山海棠对巨噬细胞上清液中炎性因子的影响,为昆明山海棠的临床应用提供实验依据。方法 收集5批国内昆明山海棠药材,提取分离得醇提物,测定其作用于小鼠骨髓来源巨噬细胞(BMDM)的半数抑制率(IC50),采用Elisa法检测各组细胞上清液中白细胞介素(IL)-10、IL-6、诱导型一氧化氮合酶(iNOS)水平。结果 与柳氮磺吡啶组比较,湖南岳阳产、浙江新昌产昆明山海棠醇提物巨噬细胞上清液中IL-6[(4.22±0.38)pg/ml、(4.55±0.44)pg/ml比(7.92±0.84) pg/ml]、iNOS[(0.07±0.04)ng/ml、(0.28±0.10)ng/ml比(0.86±0.13)ng/ml]水平降低(P<0.05),贵州黔东南自治州、福建三明产昆明山海棠醇提物的巨噬细胞上清液中IL-10[(19.34±6.06)pg/ml、(24.34±3.03)pg/ml比(9.06±0.40)pg/ml]水平升高(P<0.05)。结论 湖南岳阳产、浙江新昌产昆明山海棠的醇提物对巨噬细胞的总体抗炎功效优于柳氮磺吡啶,可作为治疗类风湿关节炎的“道地药材”深入研究。

Abstract: Objective To observe the effect of different regions of tripterygium hypoglaucum (Lévl.) hutch on macrophage inflammatory factor, and to providetheoretical basis and experimental basis for the clinical application of tripterygium hypoglaucum (Lévl.) hutch. Methods 5 batches of tripterygium hypoglaucum (Lévl.) hutch were collected, then the samples turned into alcohol extract by extraction and isolation. The IC50 values of alcohol extracts were measured by MTT in BMDM cell. B MDM cell were induced by the 5 batches of samples with IC50, then IL-6, IL-10, iNOS were detected by Elisa. The efficacy of different regions of tripterygium hypoglaucum (Lévl.) hutch on macrophage inflammatory factor was evaluated by comparison with sulfasalazine. Results The content of IL-6 (4.22 ± 0.38 pg/ml, 4.55 ± 0.44 pg/ml vs.7.92 ± 0.84 pg/ml) and iNOS (0.07 ± 0.04 ng/ml, 0.28 ± 0.10 ng/ml vs. 0.86 ± 0.13 ng/ml) in HuNan and ZheJiang groups were significantly lower than sulfasalazine (P<0.05), and the content of IL-10 (19.34 ± 6.06 pg/ml, 24.34 ± 3.03 pg/ml vs. 9.06 ± 0.40 pg/ml) in Guizhou and Fujian groups were higher than sulfasalazine (P<0.05). Conclusion The anti-inflammatory effect of HuNan and ZheJiang’s tripterygium hypoglaucum (Lévl.) hutch treat rheumatoid arthritis is better than sulfasalazine, so theyaregenuine regional drug in the treatment of rheumatoid arthritis. Additional research will analyze associations between tripterygium hypoglaucum (Lévl.) hutch and rheumatoid arthritis.

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