[论著]甘草水提物多成分肝肠吸收代谢差异研究

Time:2016-02-22 19:46:34 来源:100102 北京中医药大学中药学院[张雷(2012级硕士研究生)、刘洋、唐明敏、隗丽、侯成波(2013级硕士研究生)、程笑、王豪(2011级本科生)];100700北京,中国中医科学院中医药信息研究所办公室(潘艳丽)等 作者:张雷 潘艳丽 刘洋 唐明敏 隗丽 侯成波 程笑 王豪

《国际中医中药杂志》[ISSN:1673-4246/CN:CN11-5398/R]

卷: 37 期数: 2015年07期

页码: 636-640

栏目: 论著

出版日期: 2015-07-10

文章信息/Info

Title:

The absorption and metabolism differences of intestine and liver for multicomponent licorice water extract

作者: 张雷 潘艳丽 刘洋 唐明敏 隗丽 侯成波 程笑 王豪

100102 北京中医药大学中药学院[张雷(2012级硕士研究生)、刘洋、唐明敏、隗丽、侯成波(2013级硕士研究生)、程笑、王豪(2011级本科生)];100700北京,中国中医科学院中医药信息研究所办公室(潘艳丽)

Author(s): Zhang Lei' target="_blank" rel="external">

Zhang Lei*;  Pan Yanli;  Liu Yang;  Tang Mingmin;  Wei Li;  Hou Chengbo;  Cheng Xiao;  Wang Hao.

' target="_blank" rel="external"> Wang Hao.

* Master Graduate Student of Grade 2012, Beijing University of Chinese Medicine, Beijing 100102, China

关键词: 甘草水提物' target="_blank" rel="external">

甘草水提物; 超高效液相色谱-串联质谱; 肝肠吸收代谢差异; ' target="_blank" rel="external">封闭肠环

Keywords: Licorice water extract' target="_blank" rel="external">

Licorice water extract;  Ultra performance liquid chromatography tandem mass spectrometry;  Absorption and metabolism differences; ' target="_blank" rel="external"> In situ closed-loop

DOI: 10.3760/cma.j.issn.1673-4246.2015.07.015

摘要:

目的 探讨甘草水提物多成分的肝肠吸收代谢差异。方法 利用液质联用技术对甘草水提物的主要成分进行鉴别,并利用在体封闭肠环模型研究大鼠小肠和肝脏吸收代谢的差异。结果 甘草水提物中共鉴别出13种主要化学成分。甘草水提物中部分成分可透过肠壁吸收,且在吸收过程中伴随着成分代谢,在血浆样品中共检测到14种新生代谢产物。肝脏可代谢甘草水提物中多种成分,且较肠吸收代谢过程产生更多的代谢产物。结论 肝脏是甘草水提物多成分代谢的主要器官,肠道具有一定代谢作用。小肠和肝脏存在明显的吸收代谢差异。

Abstract:

Objective This paper was to study the absorption and metabolism differences of intestine and liver for multicomponent licorice. Methods The components were identified with the UPLC-MS/MS. In situ closed-loop method was used to carry out the comparative experiments of absorption and metabolism differences between intestine and liver. Results 13 components were identified by UPLC-MS/MS. The absorption and metabolism results indicated some components in licorice water extract could be absorbed into blood and metabolism happened during this process. 14 metabolites were detected in the plasma sample. The hepatic metabolism results indicated many components could experience complex metabolism and more metabolites could be generated. Conclusions Liver was the major metabolism organ for licorice water extract and some components could be metabolized along with the absorption process in intestine. The absorption and metabolism differences between intestine and liver were significant.

参考文献/References:

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